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KMID : 1189420230360010040
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2023 Volume.36 No. 1 p.40 ~ p.49
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Studies on Skin Anti-aging Effect of Chungpyesagan-tang(CPSGT)
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Jeon Bo-Hee
Song Young-Chae
Kim Hee-Taek
Kim Yong-Min
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Abstract
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Objectives : This study was conducted to confirm the skin anti-aging effect of Chungpyesagan-tang(CPSGT) extract.
Methods : We performed MMT assay to confirm the cytotoxicity of CPSGT. After inducing matrix metalloproteinase-1(MMP-1) with tumor necrosis factor-α(TNF-α), we investigated mRNA expression and protein secretion of MMP-1 by real-time RT-PCR and ELISA. In addition, we measured the protein expression of mitogen-activated protein kinases(MAPKs) and transcription factors by western blot.
Results : CPSGT was not cytotoxic at 25-800㎍/㎖. The mRNA expression and protein secretion of MMP-1 decreased when treated with CPSGT. The protein expression of p-ERK, p-JNK, p-p38 was decreased by CPSGT.
In addition, the protein expression of p-c-jun and p-NF-κB, which are transcription factors, were also decreased.
Conclusion : This suggests that CPSGT can inhibit MMP-1 and thus be a potential anti-aging substance.
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KEYWORD
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Anti-aging, Chungpyesagan-tang, TNF-α, MAPKs, MMP-1
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